Fluorescent Peptide Synthesis

Fluorescently labeled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorochrome-conjugated peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. Pepscan applies a wide variety of fluorescent labels. These labels can be incorporated at any desired position within your peptide.

Introduction

Fluorescently labeled peptides are frequently used for in vivo biomedical imaging, protein binding and localization studies. Fluorochrome-conjugated peptides can be visualized by fluorescence microscopy or other fluorescence visualization techniques. Pepscan applies a wide variety of fluorescent labels. These labels can be incorporated at any desired position within your peptide.

Fluorescent dyes are the primary means of labeling biomolecules. The most common such dyes are FITC derivatives, which have a wide range of applications in fluorescence microscopy, flow cytometry, and  immunofluorescence-based assays.

Fluorescent Cyclic Peptides

Fluorescent Peptides & Dye Labeled Peptides

Dye and fluorescent labeled peptides are commonly used for protein binding or localization studies.

These labels can be incorporated at many positions in the fluorescent peptide or labeled peptide. Usually, they are incorporated at the N-terminus or at Cys/Lys side chains. Examples of frequently used fluorescent dyes and fluorescence labels and common attachment options for fluorescent labeled peptides are listed below.

FLUORESCENT PEPTIDES & DYE LABELED PEPTIDES

Custom FRET Peptides Labeling

FRET-pair-combinations

FRET-pair-combinations

Common FRET Labels and their abbreviations

Common FRET Labels and their abbreviations

Amine Reactive Labeling

Amine Reactive Labeling from Kirkland Peptide provides a wide variety of fluorescent labeled peptides. The vast majority of labels are designed for amino acid labeling. Therefore, peptides can be labeled with an N-terminal free amine, internal sequence via amino acid side chain such as Lysine or Dap, or at the C-terminus of the peptide through the side chain of a lysine residue. Use the wavelength spectrum below to locate the label  equirements for your research.

Red Lapels

  • 5-carboxytetramethylrhodamine [5TMR] 
  • 5.6-carboxytetramethylrhodamine [5(6)TMR] 
  • 6-carboxytetramethylrhodamine [6TMR] 
  • Lissamine Rhodamine B [LRhodB]

Green Labels

  • 5-carboxyfluoresceine [5FAM] 
  • 5,6-carboxyfluoresceine [5(6)FAM] 
  • 6-carboxyfluoresceine [6-FAM] 
  • Methoxy coumarin acetic acid

Other Fluorescence Labeled Peptide probes also available such as

  • FITC/5-FAM (N-Terminal, Y/N Ahx)
  • MCA (N-Terminal)
  • HYNIC (N-Terminal)
  • DTPA (N-Terminal)
  • Texas Red
  • Bodipy
  • Aminocoumarin
  • and many others…
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