Sequence 3 letters:

H-Cys-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Amidation

Sequence:

H-CYGRKKRRQRRR-Amidation

Cas No. :

Molecular Formula:

C₆₇H₁₂₄N₃₄O₁₄S

Molecular Weight:

1661.98

Description

TAT (48–60), GRKKRRQRRRPPQ, is an arginine rich peptide that can cross the cellular membranes and translocate into the nucleus due to a nuclear localization signal within its sequence. TAT has been conjugated to various active agents to mediate their delivery into the cells. Tan et al. utilized TAT-derived peptides (P1, P2, P3) coupled to an anti-HER2 peptide mimetic (AHNP) and STAT3 inhibiting peptide (P1/P2/P3-AHNPSTAT3) to enhance the TAT-dependent internalization and delivery of the therapeutic peptide into different HER2-overexpressing breast cancer cells including MDA-MB-435, SKBr3, and BT-474. The P1 variant (YGRKKRRQRRR) showed highest internalization compared to the other two truncated variants (P2; YGRKKRRQRR) and (P3; YGRKKRRQR) in HER 2-overexpressing SKBr3 cells indicating that arginine residue (R) is important for the increased cellular internalization.

Reference

• Bright R. et al. Neurosci lett. 441, 120-124 (2008)
• Moultin HM. Et al. Bioconj Chem. 15(2):290-299 (2004).