Sequence 3 letters:

H-Thr-Phe-Phe-Arg-Leu-Phe-Asn-Arg-Ser-Phe-Thr-Gln-Ala-Leu-Gly-Lys-Gly-Gly-Gly-Lys-Asn-Leu-Arg-Ile-Ile-Arg-Lys-Gly-Ile-His-Ile-Ile-Lys-Lys-Tyr-Gly-Gly-Gly-Cys-OH

Sequence:

H-TFFRLFNRSFTQALGKGGGKNLRIIRKGIHIIKKYGGGC-OH

Cas No. :

Molecular Formula:

C₁₉₉H₃₂₃N₆₁O₄₇S

Molecular Weight:

4354.12

Description

Streptococcus mutans. Bacterial resistance is becoming a global issue due to its rapid growth. Potential new drugs as antimicrobial peptides (AMPs) are considered for several decades as promising candidates to circumvent this threat. Nonetheless, AMPs have also been used more recently in other settings such as molecular probes grafted on biosensors able to detect whole bacteria. Rapid, reliable and cost-efficient diagnostic tools for bacterial infection could prevent the spread of the pathogen from the earliest stages. Biosensors based on AMPs would enable easy monitoring of potentially infected samples, thanks to their powerful versatility and integrability in pre-existent settings. AMPs, which show a broad spectrum of interactions with bacterial membranes, can be tailored in order to design ubiquitous biosensors easily adaptable to clinical settings. This review aims to focus on the state of the art of AMPs used as the recognition elements of whole bacteria in label-free biosensors with a particular focus on the characteristics obtained in terms of threshold, volume of sample analysable and medium, in order to assess their workability in real-world applications.

Reference

• Pardoux É, Boturyn D, Roupioz Y. Antimicrobial Peptides as Probes in Biosensors Detecting Whole Bacteria: A Review. Molecules. 2020 Apr 24;25(8):1998.
• Lillehoj, P.B.; Kaplan, C.W.; He, J.; Shi, W.; Ho, C.-M. Rapid, Electrical Impedance Detection of Bacterial Pathogens Using Immobilized Antimicrobial Peptides. J. Lab. Autom. 2013, 19, 42–49.