Sequence 3 letters:

H-Met-Val-Arg-Arg-Phe-Leu-Val-Thr-Leu-Arg-Ile-Arg-Arg-Ala-Cys-Gly-Pro-Pro-Arg-Val-Arg-Val-OH

Sequence:

H-MVRRFLVTLRIRRACGPPRVRV-OH

Cas No. :

Molecular Formula:

C₁₁₆H₂₀₇N₄₃O₂₄S₂

Molecular Weight:

2652.28

Description

The tumor suppressor p14ARF is widely deregulated in many types of cancers and is believed to function as a failsafe mechanism, inhibiting proliferation and inducing apoptosis as cellular response to a high oncogene load. A 22-amino-acid-long peptide derived from the N-terminal part of p14ARF, denoted ARF(1–22), which has been shown to mimic the function of p14ARF, has cell-penetrating properties. This peptide is internalized to the same extent as the cell-penetrating peptide (CPP) TP10 and dose-dependently decreases proliferation in MCF-7 and MDA MB 231 cells. Uptake of the ARF(1–22) peptide is associated with low membrane disturbance, measured by deoxyglucose and lactate dehydrogenase (LDH) leakage, as compared to its scrambled peptide. Also, flow cytometric analysis of annexin V/propidium iodide (PI) binding and Hoechst staining of nuclei suggest that ARF(1–22) induces apoptosis, whereas scrambled or inverted peptide sequences have no effect. The ARF(1–22) peptide mainly translocates cells through endocytosis, and is found intact inside cells for at least 3 hours.

Reference

• Johansson HJ, El-Andaloussi S, Holm T, Mäe M, Jänes J, Maimets T, et al. Characterization of a novel cytotoxic cell-penetrating peptide derived from p14ARF protein. <em>Mol Ther</em>. 2008;16(1):115-23