Sequence 3 letters:

H-Cys-Asn-Cys-Lys-Ala-Pro-Glu-Thr-Ala-Leu-Cys-Ala-Arg-Arg-Cys-Gln-Gln-His-Amidation

Sequence:

H-CNCKAPETALCARRCQQH-Amidation

Cas No. :

Molecular Formula:

C₇₉H₁₃₅N₃₁O₂₄S₄

Molecular Weight:

2027.36

Description

H-Cys¹-Asn-Cys³-Lys-Ala-Pro-Glu-Thr-Ala-Leu-Cys¹¹-Ala-Arg-Arg-Cys¹⁵-Gln-Gln-His-NH₂ (disulfide bonds: 1-11, 3-15)The apamin sequence has been synthesized by the solid-phase procedure. The synthetic peptide showed the same physicochemical and chemical properties as natural apamin in the following tests: paper electrophoresis, amino acid analyses after acid and enzymatic hydrolyses, sequence analysis, electrophoreses after tryptic and chymotryptic digestions. Synthetic apamin was as active as natural apamin in the neurotoxic assay in mice (LD50, after subcutaneous injection, for the 20-g mouse: 58 μg).

Reference

• Sandberg BE, Ragnarsson U. Solid phase synthesis of apamin, the principal neurotoxin in bee venom. Isolation and characterization of acetamidomethyl apamin. Int J Pept Protein Res. 1978 Mar;11(3):238-45. PMID: 649258.